Sample requirements
General sample submission criteria
- SamplesÌýshould be intact i.e. not degraded.
- SamplesÌýshould be free of contaminants:Ìý260:280 1.8-2.2 and 260:230 ratio >1.8.
- RNA samples must be free of genomic DNA contamination.
- DNA samples must be free of RNA contamination.
- DNA concentration shouldÌýbe measured using the Qubit or Picogreen assay as quantification by spectrophotometric methodsÌýis inaccurate.
Application specific sample submission criteria
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³§±ð°ù±¹¾±³¦±ðÌý Amount requested Concentration Minimum volume PCR-free sample prep
Ìý1 ug DNA 30-60Ìýng/ul 30 ul PCR-plus sample prep
500ng DNA 20-25 ng/ul 25Ìýul -
³§±ð°ù±¹¾±³¦±ðÌý Amount requested Concentration Minimum volume mRNA seq
>1.25 µg total RNA 25-70 n²µ/µ±ô 50Ìýul Total RNA seq -Ìýhuman, mouse, rat, bacteria
>500 ng total RNA 20-50 ng/ul 20Ìýul ÌýTotal RNA seq - plants
>2.5 µg total RNA 100-200 ng/µl 25 ul Total RNA seq - low input
>15 ng total RNA 1-5 ng/µl 15 µl Total RNA seq - rRNA depleted submission 120 ng rRNA depleted RNAÌý 10-20Ìýng/ul 12Ìýul small RNA*
>375 ng total RNA 25-50 ng/µl 15Ìýul >500 ng total RNA 25-50 ng/µl 20 µl Ìý
* contact us for input requirements for serum and plasma.
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- Refer to your quote for theÌýamount for this service.
- Library fragments up toÌý1kb.ÌýÌý
- Custom primers must be supplied at the time of sample submission.
- One pool per run (unless prior arrangements have been made).
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We provide various capture options requiring different input amounts.ÌýContact usÌýfor further information.Ìý
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- 15-20ul of DNA at a concentration of 5-10ng/ul.
- Quantification should be performed by a fluorescence assay (Qubit or Picogreen).
- DNA of high purity (260/280, 260/230 >~1.8).
- Samples must be amplifiable by PCR (please check this prior to submission).
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Service Amount requested Concentration Minimum volume ChIP-seq (fragment size must be in 200-700bp range) >4ng 400pg – 4ng/µl 12 µl >500 ng 25-50 ng/µl 20 µl Ìý
*For Reduced Representation Bisulfite Sequencing (RRBS) and targeted Methyl-seq please
Contact us for more information.
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- >300ng of purified plasmid DNA at a concentration of 15-50ng/ul (measured with Qubit) in nuclease free water or elution buffer.Ìý
- Minimum volume 20ul/sample.Ìý
- Plasmid size: 2-25kb. Please contact us if the plasmid is outside this size range as this may affect quantity of DNA required.Ìý
- 260/280: 1.8 - 2.0Ìý
- DNA is double-stranded and not fragmented (degraded).Ìý
- The sample does not contain a mixture of plasmids.Ìý
- The DNA is clean and does not contain contaminants that may affect prep or sequencing results.
For more information, see theÌýOxford Nanopore guideline for plasmid extraction.
Please note, submitted samplesÌýare only checked with qubit. By submitting, the client confirms that the samples conform to the above requirements.
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- Volumes and concentration depends on the application.ÌýContact usÌýfor further information or refer to your quote.
- OurÌýLong & Linked Read Sample Submission GuideÌýprovides general information on DNA/RNA quality.
- For full-length plasmid sequencing, see above.
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Axiom Genotyping:
50ul of intact genomic DNA at a concentration of 15 - 50 ng/ul.
Infinium Genotyping:
20ul of intact genomic DNA at a concentration of 40 - 60 ng/ul.
Infinium Methylation array:
45ul of intact genomic DNA at a concentration of 25 - 50 ng/ul.
Note:ÌýSamples should be resuspended in nuclease free water or low EDTA TE buffer. We can accept dried samples if plate sealing is a concern.
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Contact usÌýfor further information or refer to your quote.