Protein & Biologics Characterisation
Description
The Recombinant Products Facility (RPF) offers access to a broad range of advanced techniques and equipment to support your analytical needs. Let us help you achieve reliable and impactful results.
- In-process controls play a vital role in guiding decisions during process development - helping to define upstream and downstream parameters that ensure optimal process performance and consistent quality attributes.
- Thorough characterisation of your final product is essential for ensuring consistency and reproducible outcomes.
Below is a list of some of the quality attributes tested and analytical techniques available, contact us if you would like to have more details.
Applications
- Diagnostics
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- Drug screeningÌý
- Biomaterials
- Synthetic Biology
Quality attributes and techniques available
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- Total protein and/or DNA concentration:Ìý BCA (Bicinchoninic Acid) Assay, Bradford Assay, Qubit, A280, A260
- specific protein concentration: ELISA, UV-Vis Spectroscopy
- Virus Like Particle concentration: Nanoparticle Tracking Analysis (NTA):Ìý Nanosight NS300
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Find the ideal formulation (buffer) in which your product should be stored after purification.
For preclinical trials evaluate the long-term stability of your product.
- Nano Differential Scanning Fluorometry (nanoDSF): Prometheus-nanotemper.
Analytical (HPLC) and preparative (AKTAs) Size exclusion.
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- SDS-PAGE
- Analytical (HPLC) and preparative (AKTAs) Size exclusion
- Mass photometer- Refeyn
- Intact Mass Analysis (IMA)
- Capillary electrophoresis SDS (CE-SDS)
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- Mass photometer- Refeyn
- Intact Mass Analysis (IMA)
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- Multi-Angle Dynamic Light Scattering (MADLS®): Malvern Zetasizer Ultra™
- Transmission Electron Microscopy (TEM)
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- SDS PAGE / Western Blot
- LC/MS/MSÌý
Ìý
- SDS PAGE / Western Blot
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- Endotoxin: Endosafe® nexgen-PTSâ„¢Ìý
- Host cell DNA: qPCR
- Host Cell proteins: ELISA
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- Surface Plasmon Resonance (SPR): BiaCore T200Ìý
- Bio-Layer interferometry (BLI): BLItz
- Microscale Thermophoresis (MST): Monolith NT.115
Location
Recombinant Products Facility
Level 2 and Level 3
Biological Sciences North Building (D26)
91É«ÇéƬ Sydney, NSW 2033
Phone:Ìý02 9065 6379
Email:Ìýh.lebhar@unsw.edu.au
Hélène Lebhar
RPF Head of Facility
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Phone
02 9065 6379 -
Email
h.lebhar@unsw.edu.au
Parent facility
Explore more instruments, facilities & services
Our infrastructure and expertise are accessible to 91É«ÇéƬ students and staff, external researchers, government, and industry.