BLItz
Analysis of protein interactions with minimal sample volume
Description
BLItz interferometry-based biosensor enables label-free, real-time analysis of protein interactions with minimal sample volume. This single-channel, manually-operated instrument and is ideally suited for simple, low volume experiments and can tolerate crude samples and nanoparticles.
Specifications
- Ligand Sample (immobilized): 10 – 50 µg/ml
- Analyte: 0.01 – 100x KD (0.1 – 10x KD)
- Analyte size: ≥10 kDa
- Sample and Reagent volume: 4 μl sample volume, 250 μl reagent volume
Disposable biosensors (sensor regeneration not required) - KD range: nM to mM
Applications
- Kinetic binding: ka, kd
- Equilibrium binding: KD
- Macromolecule binding
- Macromolecule quantification in crude or purified sample
Instrument location
Molecular Surface Interaction Laboratory
Room 2002, Level 2
BABS (E26)
91É«ÇéƬ Sydney, NSW 2033
·¡³¾²¹¾±±ô:Ìýmsi@unsw.edu.au
Dr Xiaojing Huang
Senior Technical Officer
-
Email
xiaojing.huang@unsw.edu.au
Parent facility
Explore more instruments, facilities & services
Our infrastructure and expertise are accessible to 91É«ÇéƬ students and staff, external researchers, government, and industry.